Research at Hibino Lab

Cardiac tissue engineering:

Our lab is spearheading innovative developments in tissue engineering, with a primary focus on three key research areas: vascularization, endothelization, perfusion, and 3D bioprinting. These pivotal domains hold the potential to revolutionize regenerative medicine and unlock new possibilities in cardiac tissue engineering.

Vascularization is a fundamental aspect of tissue engineering, especially in the context of heart tissues. Our researchers are dedicated to advancing techniques that promote the development of functional blood vessels within engineered heart tissues. Efficient vascularization is critical for enhanced nutrient and oxygen delivery, ensuring the survival and optimal functionality of the tissues post-transplantation. Additionally, a well-developed vascular network facilitates seamless integration with the host circulatory system, leading to improved therapeutic outcomes and reduced complications.

Endothelization is another crucial area of our research, where we focus on incorporating functional endothelial layers into engineered heart tissues. These endothelial cells play a vital role in maintaining vascular homeostasis, preventing clotting, and regulating vascular permeability. By integrating these essential cells into our tissue constructs, we aim to emulate the natural architecture of blood vessels, promoting long-term stability and compatibility with the host.

Perfusion is a groundbreaking field driving tissue engineering to new heights of sophistication. Our researchers work on strategies to achieve adequate perfusion of the engineered heart tissues, simulating the dynamic flow of blood through the vascular network. This approach aims to create tissues that closely resemble native heart tissue, with enhanced contractile function and physiological response. Proper perfusion also fosters nutrient and signaling molecule transport, further promoting tissue maturation and seamless integration upon transplantation.

3D bioprinting is also a highly promising and groundbreaking approach in cardiac tissue engineering. This state-of-the-art technology empowers researchers with an unprecedented level of precision, enabling them to meticulously place cells, biomaterials, and growth factors to fabricate intricate three-dimensional structures closely resembling native heart tissue. At the forefront of this innovation, our lab is actively exploring the potential of 3D bioprinting in cardiac tissue engineering. Our process involves a meticulous layer-by-layer assembly of bioinks, specialized bioactive materials that contain living cells. By incorporating various cell types, including cardiomyocytes, endothelial cells, and vascular smooth muscle cells, we can construct multi-layered tissue structures with the potential to develop into functional cardiac tissue. The beauty of 3D bioprinting lies in its ability to recreate the highly organized cellular architecture found in the heart, a feat that traditional tissue engineering methods have struggled to achieve. The controlled and precise spatial distribution of cells allows us to closely mimic the natural arrangement within the heart. Additionally, this technology enables the integration of bioactive molecules and growth factors in specific regions of the tissue construct, promoting tissue maturation and vascularization. One particularly promising application is the creation of personalized cardiac tissue constructs, tailored to match the anatomy and pathology of individual patients, potentially improving transplantation success rates and minimizing the risk of immune rejection. Though still facing certain challenges, such as ensuring long-term tissue survival and functionality, 3D bioprinting offers immense potential in revolutionizing regenerative medicine and inspiring hope for patients with heart-related conditions worldwide.

We believe our relentless focus on vascularization, endothelization, perfusion, and the use of 3D bioprinting will lead to unprecedented breakthroughs in regenerative medicine. By continually pushing the boundaries of tissue engineering research, we strive to offer innovative solutions that positively impact countless lives, providing hope for those facing heart-related conditions. Join us on this extraordinary journey as we redefine the future of cardiac tissue engineering together.

Vascular engineering

In addition to our studies in cardiac tissue engineering, Hibino Lab recognizes the challenges of complex vascular reconstruction, especially in cases of congenital heart disease. That’s why we’re at the forefront of developing innovative solutions. Our cutting-edge approach involves patient-specific, 3D-printed, tissue-engineered vascular grafts that offer unparalleled advantages. These remarkable grafts are meticulously designed to optimize hemodynamic performance, boast excellent antithrombotic and anti-infective properties, and have the remarkable capacity to accommodate growth.

In our recent study, we conducted experiments using a porcine model to assess the impact of growth on our 3D TEVG. Employing advanced imaging techniques such as magnetic resonance imaging (MRI) and 4-dimensional (4D) flow data, we customized patient-specific 3D TEVGs tailored to the pulmonary artery. The grafts’ design process was informed by cutting-edge computer-aided tools, incorporating computational flow dynamics analysis to ensure optimal shapes, thus maximizing positive outcomes.

Our findings unveiled promising results, demonstrating that our patient-specific 3D TEVGs effectively adapt to growth while maintaining ideal flow dynamics. This adaptability significantly reduces neointimal hyperplasia and promotes appropriate neovessel formation. The potential implications of our research extend to the exciting realm of regenerative medicine, offering promising avenues for vascular reconstruction and revolutionizing cardiovascular care. At Hibino Lab, we’re driven by our commitment to advancing vascular engineering and transforming patient care for a healthier tomorrow.